Recombination of bacteriophage phi X174 by the red function of bacteriophage lambda
نویسندگان
چکیده
منابع مشابه
The three-dimensional structure of frozen-hydrated bacteriophage phi X174.
The three-dimensional structure of bacteriophage phi X174 (phi X174) was determined to approximately 2.6 nm resolution from images of frozen-hydrated 114 S particles. The outer surface of phi X174 is characterized by several prominent features: (i) 12 mushroom-shaped caps (approximately 7.1 nm wide x 3.8 nm high) are situated at each of the vertices of the icosahedral virion and extend to a max...
متن کاملRequirement of protein synthesis for bacteriophage phi X174 superinfection exclusion.
Within 5 to 10 min at 37 C, bacteria infected by bacteriophage phiX174 acquire the ability to exclude superinfecting phiX particles from reproducing themselves. The superinfecting phage are blocked at a stage prior to synthesis of the parental replicative form molecule; the superinfecting deoxyribonucleic acid remains as intact (infective) single strands. Establishment of superinfection exclusi...
متن کاملGenetic recombination in bacteriophage lambda
© The Author 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Genetic recombination in bacteriop...
متن کاملThe J gene of bacteriophage phi X174: in vitro analysis of J protein function.
The J protein of phi X174 is a small, highly basic protein and is a component of the phage capsid. We have investigated the role of J protein during single-stranded viral DNA synthesis and phage morphogenesis by using an in vitro system composed of purified viral and host components (Aoyama et al., Proc. Natl. Acad. Sci. U.S.A. 80:4195-4199, 1983). The characterization of the products made in t...
متن کاملEffects of nonhomology on bacteriophage lambda recombination.
When crosses are performed under conditions severely restricting DNA synthesis, the presence of DNA sequence nonhomologies in the lac region of lambda plac5 limits the parental material contribution to and the yield of phage recombinant in a region bracketed by the nonhomologies. These observations are consistent with the expectation of a role for branch migration in the formation of heterodupl...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.29.2.438-442.1979